Describe direct and indirect immunofluorescence.
Answer: Direct immunofluorescence detects specific bacterial antigens by using antibodies conjugated with fluorescent dyes to directly bind the bacterial antigen which then fluorescences under ultraviolet (UV) light. Indirect immunofluorescence detects serum antibodies by using known antigens to bind the antibodies which can then be detected under UV light by antihuman IgG antibodies conjugated with fluorescent dyes.
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